Permeation of thiocolchicoside from the transdermal system using franz diffusion cell and analysis by HPLC

Recently, drug release applications through the skin have become very popular. One of the most remarkable of these drug release applications is transdermal drug release systems, which are drug release methods that allow the active substance to pass into the systemic circulation through the skin or artificial membranes. In this study, the optimization conditions required for the release and permeation tests of a gel drug containing the active substance thiocolchicoside were comparatively investigated using synthetic membranes without human or animal skin. For this purpose, the permeability of the gel drug in gel form and the active ingredient thiocolchicoside was carried out using Franz Diffusion Cell. Three different synthetic membranes were used in the study: Tuffryn membrane, Cellulose-based membrane and Supor hydrophilic polyether sulfone- based membrane. As a result of the investigations, it was observed that the best synthetic membrane for the permeability of thiocolchicoside in the Franz Diffusion Cell was the Supor membrane. In addition, the method's relative standard deviation values, detection, and quantification limits were determined, and permeation studies were carried out. In this study, the correlation coefficient was found to be 0.9995, and the limits of detection and quantification were 0.026 and 0.078 μg/L. In this way, the sensitivity and reliability of the validation study were determined.