Determination and comparison of in vitro antioxidant activities and some bioactive components of Ganoderma lucidum and its commercial products

Aim: In this thesis, it was aimed that in recent years as a medicinal mushroom which is used in the field of health and especially in the treatment of cancer to determine and compare the in vitro antioxidant activities with some bioactive components of different forms of Ganoderma lucidum and its capsule forms where sold in the market. Material and Method: Ganoderma lucidum mushroom is popularly used as a tea or by drinking its extract. Capsule forms are also available on the market. Mushroom samples obtained from the manufacturer in dried form and capsule forms obtained from the market were extracted by infusion method. The antioxidant activities of the mushrooms in the ground, sliced, commercial extract and tablet forms were determined using the CUPRAC, FRAP, ABTS and DDPH method, and the total phenolic content of the mushrooms using the Folin-Ciocalteu reagent and UV-VIS spectrophotometer. Antioxidant capacities and total phenolic component amounts of different forms of Ganoderma lucidum were calculated from the absorbance / inhibition values obtained by using trolox and gallic acid standards in terms of TEAC and GAE. Findings: Among the ground and sliced samples (9,66-38,0 mmol TEAC/g sample), commercial extracts (0,3-10,91 mmol TEAC/g sample) for antioxidant activity determination; among the capsule forms, antioxidant activities (2.46-136.64 mmol TEAC / g sample); total phenolic component amounts were found to be between (29.14-116.21 mg GAE / g sample) respectively, and phenolic component amount for commercial extract (12.62 mg GAE / g sample). Results: Commercial extract of Ganoderma Lucidum has been found to contain higher antioxidant and phenolic components than milled and sliced forms. It has been observed that the antioxidant and phenolic compound contents of the capsule forms differ according to each other. It should be suggested that Ganoderma Lucidum should be preferred over synthetic products in the treatment with herbs and as a food supplement, but it can be recommended to be used under doctor control in medical treatment. Keywords: Ganoderma lucidum, antioxidant activity, CUPRAC-FRAP-TEACDPPH-Folin-Ciocalteu.