Investigation of reuse capabilities of CRM isolates used in GMO analysis in the controlled storage conditions

Although the advantages and disadvantages of genetically modified organisms (GMOs) are discussed, they are still widely produced due to their potential benefits. Detection of the presence of GMO in the products is determined by qualitative screening and typing analyzes, while quantity analyzes are carried out by quantitative tests. Certified reference materials (CRM) are used as positive controls in typing analyzes from GMO analyzes and in calibration curves in quantity analyzes. The CRMs used in the analyzes are prepared fresh each time. However, the fact that these materials are very expensive and that the prepared amount cannot be used at one time poses an important problem for the laboratories performing this analysis. As a solution to this problem, it is important to determine the possibilities of reuse of the CRM isolates remaining from the analyzes. In the freeze-thaw processes applied to deoxyribo nucleic acid (DNA), it was found that there was not enough scientific data on plant products about the degradation of DNA and the studies were found to be mostly limited to parasites, viruses and blood samples. The aim of this study was to determine the degradation of freeze-thaw application of DNA isolates obtained from CRMs used in GMO quantity analysis of plant products under freeze-thaw application under controlled storage conditions. For this purpose, DNA isolation was performed by using Eurofins Genespin Kit Method from CRMs containing soy (DP-305423-1) and corn (DAS-40278-9) gene, and freeze (-20 ± 2 ° C) thawing (22 ± 2 ° C) processes were applied to the obtained isolates at certain frequencies. GMO Quantitative analysis was performed on Applied Biosystems 7500 Fast Real Time PCR System using the European Union Reference Laboratory (EURL) methods. The obtained data were evaluated statistically according to SPSS package program and the effect of freeze-thaw application on the CRM isolates studied was insignificant.