In vitro clonal micropropagation of GF-677, AK-1 and AK-2 almond x peach hybrid rootstocks by shoot-tip culture

ABSTRACTM.Sc. THESISIn vitro CLONAL MIKROPROPAGATION of GF-677, AK-1 and AK-2ALMOND x PEACH HYBRIDROOTSTOCKS by SHOOT-TIP CULTUREŞebnem Gözde DEMİRKÖKDEPARTMENT OF HORTICULTUREINSTITUTE OF NATURAL AND APPLIED SCIENCESUNIVERSITY OF CUKUROVASupervisor : Prof. Dr. Ayzin KÜDENYear : 2006, Pages: 41Jury : Prof. Dr. Ayzin KÜDENProf. Dr. Ali KÜDENDoç. Dr. Mehmet Nuri NASThis study was carried out in Biotechnology laboratory of C.U. Faculty of Agriculture,Department of Horticulture during 2004-2006. The shoot tips of AK-1, AK-2 and GF-677 almond xpeach hybrid genotypes taken from the orchards of C.U. Faculty of Agriculture, Department ofHorticulture were used. As the experimental material the viability, shoot elongation and rooting ratesof the shoot tips were investigated .In the experiment, MS macro and micro nutrients, vitamins and Fe-Na EDTA compositionwas used. During shoot elongation and multiplication stage 0, 0.5,1, 1.5 mg/l BAP and 0.1 mg/l GA3concentrations, during the rooting stage 0, 0.5,1, 1.5 mg/l IBA, 0.1 mg/l GA3 concentrations and MSmedium with no hormones as the control were used.In this work, viability rate was found to be 91.25 % in AK-2, 88.75 % in AK-1 and85 % in GF-677; multiplication rate was 2.43 shoot/explant in AK-1 genotype, 0.88 shoot/explant inGF-677 and 0.5 shoot/explant in AK-2 genotype; rooting rate was 24.06 % in AK-1 genotype, 22.25% in GF-677 and 6.19 % in AK-2 genotype; root number per mikroshoot was 2.78 in genotypeAK-1, 1.95 root/mikroshoot in GF-677 genotype and 0.94 root/mikroshoot in AK-2 genotype.Key Words: GF-677, AK-1, AK-2 almond x peach hybrid rootstocks, shoot tip culture, micropropagationII