The Role of at Klonal Relation Establishing of Sequence Analysis of the Protein A Gene ( spa ) in Hospital Origined MDR-MRSA İsolates

Methicillin-resistant S.aureus is one of the most significant agent of community-acquired and hospital-acquired infections. The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in many countries increases consistently and, more than half of all S. aureus disease isolates are MRSA in hospitals and in some areas. MRSA strains are becoming increasingly multiresistant, and have recently developed resistance to vancomycin, usedsuccessfully to treat MRSA for more than 30 years.Finding of infections and clonal specialities, source of infections and carrier with transmission ways are important because successfully controling of MDR-MRSA and hospital-acquired infections.Reproducibility and discriminatory of phenotypic identification methods which is a typing method are poor. Also, phenotypic identification methods could not distinguish similar phenotypic strains with different genotypes. Therefore, recently, genotyping methods which have high reproducibility and discriminatory are used for identifications. PFGE which is genotypig methods have consented the ?gold standard? of molecular typing at klonal relations establishing of hospital-acquired MRSAIn this study, it has been tried for identification of at klonal relations establishing of MDR-MRSA that isolated from hospital-acquired infections in clinics of Ç.Ü. Faculty of Medicine Balcalı Hospital with PFGE and spa typing method. In this study, it has been used 27 izolates that izolated from hospital infections determined by MDR-MRSA between 2008-2009. Isolates were characterized by antibiotyping as determined by the disk diffusion method (Kirby-Bauer). Later, isolates were investigated with PFGE and spa typing metod and typed in accordance with their DNA bant profiles.As a result, of 27 MRSA isolates, 6 types were evaluated with antibiotyping and 10 type with PFGE methods. It was substantiated that all of isolates had protein A. 5 of 27 isolates were spa tip t190 and 22 isolate were spa tip t030. In typing of searching isolates, genotyping metods were found more sensitivite and it was deduced that PFGE was more importand at klonal relation establishing. It was observed that spa typing metod was better than other metods because of reproducibility, transportability and comparability of results.