Comprehensive evaluation of the phytochemical composition and pharmacological activities of hypericum perforatum L

This thesis study investigated the chemical composition and rejuvenating potential of the components of the medicinal aromatic plant Hypericum perforatum L. Samples of this potential plant were obtained from three different regions. Controlled oil extraction was performed using hydrodistillation (Clevenger apparatus) and extraction (Soxhlet system). The chemical components of the obtained oil were analyzed using GC-MS. The therapeutic significance of the identified images was evaluated by comparing them with literature data. Furthermore, FTIR analyses were performed to determine the chemical structures; SEM-EDX elemental trace analyses were recorded to determine the unit element structure. GC-MS analysis systems were classified according to the main systems; alpha pinene (34.85-2.59), germacrene D (21.23-14.57), spatulenol (8.75-2.53), octane-2-methyl (8.67-1.38), nonane-3-methyl (7.53-0.58), beta pinene (7.23-1.87), gamma murolene (5.80-0.41), beta caryophyllene (10.27-2.17%), delta kadinene (6.98-1.24%), cyclosativene (5.22%), bicyclogermacrene (5.03-3.52%), seychellene (5.06%), cyclododecane (8.19-1.92%), alpha decene (7.95%), palmitic acid (10.94-5.63%), elaidic acid (32.80-4.42%), linoleic acid The following components were identified: terpene nutrients (39.72-4.72%), oleic acid (52.23-0.49%), n-tetracosane (10.14-5.63%), octaethylene glycol (5.19-2.42%), (Z)-9,17-octadecadienal (6.78%), and ethyl oleate (11.70%). Hydrodistillation showed a high concentration of terpene nutrients, while extraction showed a high concentration of fatty acids. It was determined that sesquiterpenes and sesquiterpenoids predominated in the above-ground parts of the plants through hydrodistillation, while aliphatic hydrocarbons and fatty acids predominated through extraction; and monoterpenes predominated in the seed parts through hydrodistillation, while fatty acids predominated through extraction. FTIR analyses did not show a significant difference between molecules according to regional variation. Plants and plant parts were analyzed with SEM-EDAX, revealing approximately %97 C and O elements, and around %3 K, P, Ca, Mg, S, Si, Na, and Al elements. According to the analysis systems we obtained, the elements and molecules are commonly used in the literature for skin healing, pharmaceutical active ingredients, and some reproductive treatments.