Investigation of the impact of heavy metals (copper and lead) on the photosynthetic pigment content and antioxidant mechanism of two different bryophyte species

In this study, short term reactions caused by heavy metal administration was evaluated in light of physiological and biochemical parameters on types of Homalothecium sericeum (Hedw.) Schimp. which belongs family of Brachytheciaceae and Hypnum cupressiforme Hedw. which belongs family of Hypnaceae. The plants collected in the field work has been brought to the laboratory on the appropriate bag. After appropriate treatment of the washing operation bryophyte species prepared media were cultured in solutions containing Cu and Pb. Heavy metal deposition levels, dry weight, photosynthetic pigments analysis, hydrogen peroxide (H2O2) content determination, lipid peroxidation andantioxidant enzymes(superoxide dismutase, catalase, peroxidase, ascorbate peroxidase) non–enzymatic andantioxidant molecules (ascorbat and proline) quantity were studied for the types of bryophyte. Both type softhe metal structure are exposed is determined that accumulatein specific proportions. Activity of superoxide dismutaz and peroksidaz has been increased inspite of there is no significant change was not seen askorbat values on Cu applied samples of Hypnum cupressiforme as the cause of the decreasein the catalase enzyme activity such as catalase activityis from stopping act by plants inhibitors of non-heavy metal ions such as copper cations. Pb applied samples of Homalothecium sericeum, The absence of H2O2 accumulation of ascorbate peroxidase and catalase activities effect on pigment degredation and H2O2 detoxification or this metals can be indicator of oxidative damages that will causes excessive stress on cells. Key Words: Homalothecium sericeum, Hypnum cupressiforme, heavy metal, stres, antioxidant