IL-8 ve IGF-1 düzeylerinin Semendeki DNA fragmentasyonu ve standart sperm parametreleri üzerindeki etkisi

Since many studies have demonstrated that there is an influence on several elements of DNA fragmentation in sperm, we will discuss the effects of interleukin 8 and IGF-1 in this study. This study was conducted at Kamal Al-Samarrai Hospital in Baghdad, where 120 random samples were selected from patients to reveal the type of infertility and the reasons that were medically approved in this field. The fundamental principle of the test was carried out using a commonly available kit and the quantitative sandwich enzyme immunoassay method. The kit we are employing is based on the Enzyme-Linked Immunosorbent Assay (ELISA), a technology based on a biotin double antibody sandwich. The wells had previously been pre-coated with an IL-8 monoclonal antibody. After adding IL-8 to the wells, the plates were incubated. Next, biotin-tagged IL-8 antibodies were added so they could combine with streptavidin-HPR to form an immunological complex. The plates were then washed to remove any unbound enzymes. This was done to make sure that there were no unattached enzymes. As a result, the objective of this research was to investigate the viability of employing the insulin-like growth factor I (IGF-1) complex in order to improve the quality of sperm that had previously been incubated.