Properties of immobilized glucose oxidase and enhancement of enzyme activity
2008
0 views
0 downloads
Advisor: Prof. Dr. Selma Ateş
Abstract (EN)
Gluconic acid and derivatives are important compounds that are used in food, pharmaceutical, textile, detergent, leather, construction and other biological industries. In this study, optimum conditions for production of gluconic acid were investigated by using immobilized glucose oxidase enzyme in shake flasks. Glucose oxidase was immobilized in alginate gels with the method of entrapment. Effects of silicone oil and soybean oil on production of gluconic acid was studied. The specific activities were found 2,6x10-2 U/mg and 1,7x10-2 U/mg, for free and immobilized enzyme, respectively. When 4 % (v/v) silicone oil or soybean oil were used, the activity of immobilized glucose oxidase increased 1,69 times and 1,33 times, compared to media without these oils, respectively.
Author
Nesrin İçli
How to Cite
Nesrin İçli (Master Thesis). Properties of immobilized glucose oxidase and enhancement of enzyme activity, 2008, Gazi University.
Keywords
License
Tüm Hakları Saklıdır
This work is shared under the specified license terms.
More theses from Gazi University
- Occupational accident analysis and modelling in oil and gas drilling sector Turkey(2021)
- XVI. yüzyıl Anadolu'sunda Oğuzların Karkın Boyu(2004)
- Sharing of real life geometry samples via a social learning environment: A case study(2021)
- Evaluatıon of calcium hydroxide removal efficiency of two different irrigation activation techniques from artificial internal resorption cavities prepared at different root levels(2021)
- Experimental development of the interfacial bond-slip model between textile reinforced mortar strips and masonry walls(2025)
- The use of verbal memory in the context of sustainability and power at the museums of Turk(2010)