Investigation of muscarinicreceptors in mesenchymal stem cells obatined from different sources in vitro and influence of receptor blockadge on differentiation

Cells carry out their functional activity by communicating via endogenous substances and receptors with each other. The stem cells also transplanted so as to regeneration settled into the new tissue, to show the functional activities as required of the tissuemay be able to transport the receptors on their surface of the cells. To show thatwhichreceptors reside in themesenchymalstem cellswill be providedtouseof more convenient of the cells so as to regeneration. In this study, human placenta, fatal membrane (FM) and bone marrow (BM)derived MSCs in the1st, 2ndand 3rdpassage, osteogenic andadipogenicdifferentation,additively, in the differentiation under the effect of atropine, Mrna expression levels M1, M2, M3, M4 and M5 subtypes of muscarinic receptorswereshown by RT-qPCR. So as todetermination of the blockers the effects on differentiation from osteogenic aspectBoneMorphogenetic Protein (BMP-6) andPeroxisomeproliferator-Activated Receptor gamma (PPAR) were investigatedfromadipogenicaspectPPARγ, mRNA expressions were investigated via RT-qPCR. In our study, compared to the control groups, generally determined significantly increase on M1 mRNA expressions of FM groups.M5 mRNA expression compared to control group did notchanged significantly but in the KI groups of the samereceptorshowedsignificantly decreased. BMP-6 mRNA expressions in the FM and KI groupsof osteogenicdifferentiatedcells showedincrease significantly compare to undifferentiated cells, but did not determined significantly change theBMP-6 mRNA expressionstheeffect of atropine in the osteogenicdifferentiated cells compared to normal osteogenic differentiated cells.In the groups derived from both sourcesPPARγmRNA expressions inadipogenicdifferentiatedcellsshowed the significant increase compared to control group. Distinctly,whereas the cell group of the atropine effect in the FM groupincreasednonsignificantlycompared to normaladipogenicdifferentiatedcells, it decreased this expressionsignificantly. This results indicatethe cells obtained from the different sources in that the expressions of muscarinic receptors behave disparately and indicate that there are also some changes derived from the same sources cells according to the passage and differentiation. Therefore, thiscellsused so as to clinical purposeto use moreeffective obtaining from the different sources and from the different passages in the same sourcedemonstratetheimportance to investigate in terms of muscarinicreceptorsand the other receptors with in vivo studies.