Investigation of salt stress response of five triticale genotypes in in vitro culture using some biochemi̇cal and physiologi̇cal parameters

Salt stress is one of important matters negatively affects of agricultural productivity in worldwide. Triticale has hybrid genome, its source of high grain, good quality and resistant to pathogen. Resistance of triticale growing cultivars ( Alper Bey, Mikham 2002, Tatlıcak, Melez 2002 and Ümran Hanım) of triticale to salinity were carried out in green calli induction in this research. Mature embryo was choosed as explants for calli formation and green calli induction. Tested cultivated differed in their calli formation and green calli induction. Tatlıcak, Ümran Hanım and Alper Bey were obtained for more quality calli formation; likewise, the same cultivars observed at the more green calli induction in the NaCl stress. Seriously reduce in green calli induction was determined in in vitro media. In terms of the resistance to NaCl based on green calli, tested cultivars were observed to Tatlıcak > Ümran Hanım > Alper Bey > Mikham 2002 > Melez 2001. A significant sugar and proline were reached in tested cultivars than in non-tested cultivars when all were carry out NaCl stress. Proline amount reached at 200 mM whereas the highest and lowest amount was observed at 200–0 mM in vitro NaCl media. The amount of soluble sugars was highly related to 0–200 mM NaCl levels. While lipid peroxidation was observed to be higher in salt-sensitive genotypes than salt resistant ones, H2O2 amount increased in salt-resistant genotypes and decreased in salt-sensitive genotypes. Antioxidant enzyme activities demonstrated an increasing trend against of the high levels of salt.