Effects of treatment processes in infertile patients on embryo parameters and in vitro fertilization (ivf) results

In vitro fertilization (IVF) therapy is the most commonly used assisted reproductive techniques in infertility treatment.The success of live birth rate with assisted reproductive techniques are known about 30% for each embryo transfer.In our study, the indications and pregnancy rates of 455 couples diagnosed with infertilitywere examined.The indications of the couples included in the study were male factor, unexplained infertility and female factors, respectively.In the study, 20% of pregnancy group and 63.7% of non-pregnancy group were evaluatedaccording to their hormonal values with in vitro fertilization parameters, oocyte number, number of fertilized oocytes and embryo quality, fertilization rates and the correlation between the findings was statistically analyzed. Acquired data were analyzed by number, percentage, mean and t-test by chi-square test.When embryo quality was assessed, blastomers were graded according to a grading system, with equal size, round and clear cytoplasm, and embryos free of fragmation. When embryo quality was assessed blastomeres were classified according to equal size, round and clear cytoplasm and embryos free of fragmentation.Embryos with a fragmentation rate of 20% were grade I, embryos with increased blastomeric differences were grade II and 20% larger and 50% range embryos with fragmentation grade III, different blastomer number, shape and size, fragmentation rate was more than 50% and blastomer cytoplasms were dark and heterogeneous embryos were selected as grade IV.Follicles obtained from in vitro fertilization patients after controlled ovarian hyperstimulationwere collected by oocyte pick-up to separate the granulosa cells around the oocyte.Our results were statistically evaluated between pregnant women and non-pregnant women with hormone values associated with pregnancy.Analyzes were compared between the given protocol and fertilization. Our study shows that improves oocyte quality, leading to a higher fertilization rate. The retrospective study was carried out in terms of the applications of the drug protocols and the in vitro fertilization parameters to determine the beneficial effects of IVF treatment procedure.