Evaluating the efficiency of resveratrol at intestinal ischemia and reperfusion injury

Ischemia and reperfusion injury plays a role at the etiology of necrotising enterocolitis,invagination, inflammatory diseases of intestine, pull-through operations, strangulatedhernias, free and pedicled flaps of intestine and intestinal transplantation. The features ofresveratrol such as antioxidant activity, prevention of apoptosis and increasingproduction of endothelial nitric oxide directed us to research whether intraperitonealadministration of resveratrol has an effect on reducing organ disfunction or not. Withthis experimental study model, we aimed to evaluate the effectiveness of resveratrol atischemia and reperfusion injury.The experiment was performed with 35 adult male Wistar albino rats, weighing 180-220gram. Rats were assigned at 5 equal groups randomly. Group 1 was seperated as thecontrol group. Ischemia was applied at Group 2 and Group 3 for 30 minutes and Group 3and Group 5 for 120 minutes by occlusing the superior mesenteric artery. Afterischemia, resveratrol was administrated intraperitoneally to the Group 2 and 3 with adose of 10 ng/kg. Subsequent to the ishemia, we performed reperfusion for 60 minutesto the all groups and at the end of the experiment approximately 3 cm of terminal ilealsegment was resected for the histopathological research.Apoptosis and dispersion of TNF-?, IL-1ß, IL-6 and IL-8 was investigated at thesections histopathologically.As a result, even application of resveratrol can?t stop the mechanism of apoptosis, itreduced this mechanism a little. Also it was thougt that early phase effect was more thanlong term effect and it causes a delayed apoptosis. Again it was observed thatadministration of resveratrol was not statistically significant and this effect occuredespecially after late ischemia. We believe that resveratrol will be in clinical usage withtime after sufficient researching on its side effects and pharmacokinetics. We supposethat in the future resveratrol should be used for the purpose of prevention, particularlyfor the cases at the prematurity and newborn clinics who are under the risk of necrotisingenterocolitis.