Investigation of biochemical, hematological and behavioral responses against aclonifen administration in oncorhynchus mykiss

This investigation was conducted to evaluate the toxic effects of aclonifen, a herbicide, on oxidative stress biomarkers and biochemical indices of serum in juvenile Oncorhynchus mykiss following 24, 48, 72 and 96 hours of application to sublethal concentrations of 12.7, 63.5 and 127 μg/L. Pesticide treatment caused prominent elevation in the level of serum glucose, and in the activities of alanine aminotransferase, aspartate aminotransferase reflecting hepatotoxic potential. The application resulted in a marked induction in the activities of antioxidant enzymes which were accompanied with elevation in TBARS and protein carbonyl level reflecting loss of membrane integrity and protein function. The decrease in protein levels in gill and liver and triggered hyperglycemic condition might be proposed as general adaptive responses. The provoked oxidative stress clearly indicates the pro-oxidant potency of aclonifen. Considering the results, it can be concluded that aclonifen treatment resulted in tissue-specific adaptive responses to handle the stressful condition and observed early response in brain shows its sensitivity possibly due to high lipid content and low antioxidant capacity.