Isolation and molecular diagnosis of Trichoderma spp. and evaluation of it's inhibitory effectiveness on some pathogenic fungi

In this study.4 isolates of Trichoderma spp. as well as 7 isolates of Macrophomina .phaseolina and one isolate of Fusarium were isolated and identified from soil and compost in Adana province/ Turkey. In addition Two isolates Dermatophytes sp, were included for molecular study.The identification of Trichoderma spp was carried out based on morphological features as well as phylogenetic analysis using two primers (ITS1 and ITS4). Isolates were identified as T. viride, T. virens,T. harzianum and T. lixii. while all isolates of M.phaseolina were identified as M.phaseolina based on morphological features as well as phylogenetic analysis using two primers (ITS5 and ITS4 ). Also isolates of Fusarium and dermatophytes were identified as F.oxysporum, Trichophyton tonsurans and Microsporium canis respectively based on phylogenetic analysis using two primers (ITS1 and ITS4 ). From the antagonistic test. all isolates of Trichoderma showed promising results in reducing growth of pathogens under in vitro conditions. However, the efficacy was variable among the tested isolates.The best antifungal concentration was (15 ml/L) with T. harzianum isolate in inhibition for M.phaseolina, F.oxysporum, Trichophyton tonsurans and Microsporium canis.