Determination of microbial allergens from biological risk factors and risk analysis in feed factories around Izmir province

The aim of this thesis is to determine the microbial concentration in the air of feed mills, which is one of the very rich occupational environments in bioaerosol, to determine the percentage of Aspergillus, Penicillium, Alternaria and Cladosporium genera related to allergic reactions and to perform risk analysis by 5x5 L-Type matrix method. In this context, bacteria and molds were samples collected with Eco-Mass 100 Air Sampling Device. 9,4×103 cfu/m3 (9382 colonies) bacteria and 9,4×103 cfu/m3 (9412 colonies) fungi colonies were isolated. The distribution of 4 allergen genera identified according to the number of colonies were 4489 colonies Aspergillus spp. (%47.7), 2179 colonies Cladosporium spp. (%23.2), 795 colonies Alternaria spp. (%8.5), 590 colonies Penicillium spp. (%6.3) and 1359 colonies others (%14.4). In this study, the dominant genus was determined as Aspergillus spp. in all 3 feed factories. Biological risk factors were determined and risk analysis was performed with 5x5 L-Type Matrix.