Identification and determination of antibiotic resistance of Salmonella species isolated from İzmir Gulf

ABSTRACT ARKAN DEMİRCAN, Beril MSc in Biotechnology. Supervisor: Prof. Dr. İhsan Yaşa February 2016, 84 pages In this study, a total of 75 sea water samples obtained from different places of coastal areas of the Gulf of Izmir and river basins which flow into the called as inner and middle areas of the gulf were analyzed for presence of Salmonella sp. by using the ISO 6340:1995 standard. As a result of API 20E test, 4 of the (5.3%) 75 different samples were identified as Salmonella sp. After the isolations, antibiotic resistance tests were perfomed according to the Kirby-Bauer disk diffusion method. All strains were determined that resistant to at least 5 or more antibiotics. By using moleculer methods in chromosomal and plasmid DNA of antibiotic resistance strains of Salmonella, conserved sequences, Class 1 integron analysis (CS and int genes) and 9 antibiotic resistance genes (Sulfonamide, ampicillin, aminoglycosides 1-2, trimethoprim, tetracycline, florfenicol, ofloxacin and gentamicin) were analyzed. Our strains were determined resistant to 6 of the examined antibiotics (tetracycline, sulphonamide, aminoglycosides, chloramphenicol, ofloxacin and gentamicin) in this study and these resistance originate both chromosomal and plasmid. Antibiotic groups, which have the most resistance of 4 strains, were determined as respectively, aminoglycosides, chloramphenicol, sulphonamide and ofloxacin. Key words: Salmonella, multiple drug resistance, Class 1 integron, plasmid, Gulf of Izmir