Antioxidant system in liver tumor and the effect of colchicine on liver tumor

ABSTRACT Antioxidant system in fiver tumor and the effect of colchicine on liver tumor Hepatocellular carcinoma(HCC) is representing the third largest cause of cancer related death and its incidence is increasing day by day. It would be reasonable to expect that oxygen radicals would likely persist longer under conditions of oxidative stress presenting an increased risk of oxidative damage to DNA Colchicine is an ancient drug, widely use in the the treatment of gout, Behcet's disease(BD), Mediterranean fever (FMF), liver cirrhosis, dermatologic disorders and scleroderma. Increased neutrophil functions and excessive production of reactive oxygen species(ROS) were claimed some of these diseases that colchicine is used for treatment. In this study we aimed to study antioxidant system and malondialdehyde (MDA) in HCC and also effect of colchicine on HCC, an antimitotic and microtubulin formation disturbing drug. For this purpose we developed an experimental HCC model by using N-nitrosodiethylamine (DEN), a chemical carcinogenic agent. The activities of antioxidant enzymes including glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and a well-known oxyradical scavenger reduced glutathione (GSH) and MDA one of the end product of lipid peroxidation were determined in erythrocytes and fiver. Also ALT, AST, ALP and total protein were determined in serum. GSH-Px enzyme activity and level of GSH were assayed according to Beutler methods. SOD enzyme activity and MDA content were assayed according to Mc Cord and thiobarbituric acid methods, respectively. Histopathologic investigations were performed light microscopy. In the light of the results, it was concluded that free radicals in fiver may be the one of the reason for the formation of HCC. Colchicine inhibit formation of HCC but this effect wasn't occured by antioxidant system. Key Words: Hepatocelluler carcinoma, Colchicine, Free radicals, Antioxidants, N-Nitrosodiethylamine. xii