Different methods on haploid embryo rescue obtained using irradiated pollen
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Abstract (EN)
This study was conducted between the years 2007-2009 in Horticulture Department, Faculty of Agriculture, University of Çukurova. Genotype Y2 and Y3 selected from backcross three (BC3) population of Yuva melon genetic resources collected on the Department of Horticulture are allocated. In this study, different methods for embryo rescue were tested to find an easier, fast, economical and effective method. ?Inspecting the seeds one by one? was used as control treatment. Other four methods tested are ?sowing seeds direct nutrient media?, "inspecting seeds on the light", "floating seeds on liquid media" and " floating seeds on liquid media after surface sterilization?. Overcome of this study show that, there is no significant difference statistically between the methods ?Inspecting the seeds one by one?, ?sowing seeds direct CP nutrient media? and "inspecting seeds on the light" although the average number of embryos per fruit are slightly different (3.3, 2.4 and 3.1 respectively). No embryo production was obtained from liquid culture due to infection. When labor costs and time requied for embryo rescue taking into account, the best methods are ?sowing seeds direct CP nutrient media? and "inspecting seeds on the light".
Author
Gökhan Baktemur
How to Cite
Gökhan Baktemur (Master Thesis). Different methods on haploid embryo rescue obtained using irradiated pollen, 2009, Çukurova University, Ziraat Bölümü.
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