Synthesis, characterization and biological activity schiff base and metal complexes obtained from chemotherapeutic 5-fluorouracil

Studies on derivatization of drugs and synthesis of metal complexes in order to increase the effectiveness of chemotherapeutic drugs and reduce their side effects are among the popular topics of today. In this study, Schiff-based Iron (II), Nickel (II) and Ruthenium (II) metal complexes derived from 5- fluorouracil were synthesized and using various spectroscopic methods (elemental analysis, FT-IR, UV-Vis., 1H and 13C NMR, mass spectrometry, thermal analysis (TGA) and magnetic susceptibility) were characterized. When the molecular structures of the complexes were examined, it was determined that they had octahedral geometry. According to the results of the spectroscopic data, Fe(II) and Ni(II) complexes are coordinated to the C=O, NH2, NH and C=N groups of the Schiff base ligand, while the Ru(II) complex is the ligand C=O, NH2, and C=N It was observed to bind to N groups. Molecular formulas of the complexes can be defined as [FeL(H2O)2]Cl.4H2O, [NiL(H2O)2]Cl∙5/2H2O and [Ru(p-simen)L]Cl2.H2O. In addition, all compounds were examined for their antioxidant and antimicrobial activities. According to the antioxidant activity results, while the ligand and its metal complexes exhibited considerably higher activity than 5-Fluorouracil, the antiradical activity of the 5-FU drug was determined to be very low. In addition, the antioxidant activity of Ru(II) metal complex was generally higher than other metal complexes and ligands. According to the antimicrobial activity results, it was determined that it showed better activity than the compounds synthesized from 5- FU. In addition, the activities of 5-FU drug and its ligand and metal complexes on pBR322 plasmid DNA were investigated using gel electrophoresis method. When the activity of the compounds on DNA was examined, it was seen that the ligand and 5-FU did not cause breakage in the single chain of DNA. Considering all the results of the research, it is thought that ligand and metal complexes will shed light on the synthesis of new generation drugs as a result of antioxidant and anticancer studies with new in vivo studies. Year 2021, 94 Pages Keywords: Schiff Base, Metal complexes, 5-Flurouracil, Antioxidant and Antimicrobial activities, DNA activity