Evaluation of the genotoxic and antigenotoxic effects of corilagin against mitomycin-C

There are many secondary metabolite types with organic structure in the organs of plants such as roots, stems, leaves and fruits. It has been reported in many studies that these compounds have protective and preventive effects against genetic damage caused by free radicals, and studies on the antioxidant effects of these natural compounds are still ongoing. In this area, intensive research is carried out especially on polyphenols and mostly human peripheral lymphocyte cells are used for in vitro studies. Corilagin, a tannin from the group of polyphenolic compounds, was chosen for use in this study. Korilagin is a compound that has a wide variety of pharmacological effects and is found in many species of plant families such as Euphorbiaceae, Geraniaceae and Lythracea. In this thesis study, the genotoxic and antigenotoxic effects of corilagin against mitomycin-C (MMC) in human peripheral blood lymphocyte cells were investigated. Both genotoxic effects and antigenotoxic effects of corilagin against MMC, which causes oxidative damage and is known to have genotoxic effects, at doses that do not show cytotoxic effects, were evaluated by micronucleus (MN) and chromosome aberration test methods. This study was conducted repeatedly with blood samples taken from 3 different donors. A negative control group without test substances and a positive control group with 0.2 µg/ml MMC were used. In the treatment groups, 10, 25, 50, 100 µg/ml corilagin was added alone and simultaneously with 0.2 µg/ml MMC. Two slides were prepared from each sample and the results were examined by applying the micronucleus test protocol and chromosome aberration test protocol. As a result of the experiments and counts, it was determined that corilagin did not cause a statistically significant increase in the MN and CA frequency in the groups treated with 10, 25, 50 µg/ml concentrations, and it was also determined statistically significant decrease in the MN frequency and chromosome abnormalities caused by MMC treatment. At a concentration of 100 µg/ml, it was observed that the damage increased and the prooxidant effect was observed. The results show that corilagin has a dose dependent antigenotoxic effect on genetic damage caused by MMC.