Investigation of the properties of the coelom fluid obtained from the red earth worm Eisenia fetida

Earthworms are critical to the soil ecosystem and are called ecosystem engineers because they improve soil structure and production. Eisenia fetida culture worms, also known as the red California worm, are the most suitable worms for vermicompost production because of their rapid reproduction and easy production. The coelomic fluid they secrete help their physiological activities. This liquid, which they release during stress, also has a significant effect on soil health and ecotoxicity. In this study, some biological properties of coelomic fluid obtained from E. fetida were investigated. For this purpose, 3 bacteria were selected among the bacteria isolated from coelomic fluid, they were identified based on the 16S rRNA sequence and 2 of these were similar to Bacillus cereus and 1 to Bacillus thuringiensis. When the enzymatic activities in the coelomic fluid were controlled, the presence of lipase, cellulase, amylase, protease, phytase and arylsulfatase activities were detected. The antibacterial effects of the coelomic fluid was investigated against E. feacalis, E. coli, S. epidermidis, S. aureus, K. pneumonie, S. typhimurium, Y. enterocolitica and P. Aeruginosa. According to the results, it showed inhibitory effects on test bacteria. Additionally, The Thin Layer Chromatography results showed the presence of glucose and maltose in the coelomic fluid. Protein pattern of fluid showed five clear protein bands in the range of 17–46 kDa. With this study, some biological properties of coelomic fluid have been revealed, and will shed light on future studies