Determination of oxidative stress and cellular immunity in patients with diabetic nephropathy and non-diabetic which hemodialysis in treatment due to chronic renal failure

Chronic renal failure (CRF) may occur for many reasons and diabetes mellitus is one of the most important causes. Acute and/or chronic complications were seen in result of diabetes mellitus developing. Macrovascular and microvascular complications are develop from chronic complications. Diabetic nephropathy is one of microvascular complications. In these patients, the oxidative stress is one of the mechanisms proposed to explain the loss of progressive function. In addition, in these patients, the immune disorders due to various reasons observed. In this study aimed to evaluate of oxidative stress [8-hydroxydeoxyguanosine (8-OhdG), malondialdehyde (MDA)], endothelial damage [asymmetric dimethylarginine (ADMA)] and markers of cellular immunity [interlokine-6 (IL-6), tumor necrosis factor- ? (TNF-?), neopterin (NP) and high sensitve C reactive protein (hsCRP)] in patients with diabetic nephropathy and non-diabetic treated with hemodialysis because of chronic renal failure. Determination of 8-OHdG levels used by Enzyme immun assay (EIA) method. MDA, ADMA and NP levels were determined by using high performance liquid chromatography. IL-6 and TNF-alfa levels were determinated by ELISA method and Hs-CRP values were measured with nephelometric method. 8-OHdG and MDA levels were observed statistically increased when compared to control in both patients groups after dialysis. ADMA and NP levels were observed statistically decreased when compared to control in both patients groups after dialysis. IL-6 and TNF-? levels were found increased when compared to controls in all patients groups before dialysis. As a result, oxidative stress in patients with diabetic nephropathy treated with hemodialysis due to CRF was higher than stress in non-diabetic patients treated with hemodialysis due to CRF. In contrast, inflammation in patients with non-diabetic patients was found to be higher than in patients with diabetic nephropathy.