Kronik yaralarda miRNA'larin rolü

Chronic wounds, especially diabetic foot wounds, cause significant loss of workforce and impose a significant financial strain on the medical system in all countries. Diabetic foot ulcers are a primary contributor to leg amputations globally. Small, non-coding RNA molecules known as miRNAs control post-transcriptional levels of gene expression and are becoming more and more significant in the control of wound healing processes. This study examined the function of the miRNA-29 family in the pathophysiology of diabetic foot wounds, which are classified as chronic wounds. The study included a total of 16 individuals, comprising eight with diabetic foot wounds and eight control individuals with acute surgical wounds. RNA was isolated from tissue biopsies taken from the patients, and then the levels of miRNA-29a-5p, miRNA-29b-1-5p, and miRNA-29c-5p were evaluated using the real-time PCR method. The data were statistically analyzed using non-parametric tests and ROC curve analysis, and expression levels were normalized with the RNU6 reference gene. The results indicated that the expression of miR-29a in diabetic foot wounds was substantially reduced in comparison to the control group (p=0.0087) and that the AUC value in the ROC analysis was 0.89, indicating a highly discriminatory potential. In contrast, it was determined that miR-29b and miR-29c did not show significant differences in tissue. It is widely recognized that miR-29a is involved in numerous critical biological processes, including immune regulation, angiogenesis, and fibrosis. In conclusion, this thesis emphasizes the fundamental role of the miRNA-29 family, particularly miR-29a, in the pathogenesis of chronic wounds, specifically diabetic foot wounds. The results indicate that miR-29a has the potential to serve as a tissue-based biomarker and that it may be a targetable therapeutic agent in the future.