Küçük hücreli olmayan akciğer kanserine duyarlılıkta KLK10 geni (RS7259451) polimorfizminin rolünün araştırılması

Nowadays, with the COVID-19 disease, the significance of lung cancer specially NSCLC has increased. Moreover, it is also important because it is the second most deadly cancer among other cancers. Kallikrein 10 (KLK10) is one of 15 kallikrein family members. It is a serine endopeptidase and also it has a potential of tumor suppressor protein in prostate and prostate malignant cells. The aim of this study, observation of the impact of KLK10 gene (rs729451) in NSCLC. Blood sample were collected from 48 NSCLC patient and 46 healthy control groups. All DNA isolated from blood samples by using DNA Isolation Robot. Then DNA samples were prepared to make Real-Time PCR (RT-PCR) by using KLK10 detection kit. After RT-PCR, allelic discrimination was obtained from both patient and control groups. Then statistical analyzes were done in SPSS 26.0 by using Fisher's Exact Tests and Chi-square tests and also student's t-test was utilized to get numerical values. P values less than or equals to 0.05 are considered statistically significant. According to analysis, GG wild type homozygote 52.1% (n=25), GT heterozygote 45.8% (n=22) and TT 2.1% (n=1) has been recorded in patient. In addition to this, GG wild type homozygote 60.9% (n=28), GT heterozygote 32.6% (n=15) and TT 6.5% (n=3) has been obtained in control group. Moreover, G allele 80.2% (n=72) and T allele 19.8% (n=24) in patient, and G allele 77.2% (n=71) and T allele 22.8% (n=21) in control group. In the control group, the TT genotype was three times greater (p=356). These findings suggest that the TT genotype is a carrier genotype. Nonetheless, the number of TT genotypes (both control and patient) was extremely low, requiring more research. As a consequence, there is no statistically significant difference in KLK10 polymorphism between the control and patient groups (rs7259451). It's possible that the TT genotype is a carrier gene. More research, on the other hand, is already required. Perhaps this study might be improved by include a broader or diverse population. Otherwise, alternative SNPs in this gene can be investigated. Keywords: Non-small cell lung cancer, NSCLC, polymorphism, KLK10, rs7259451