Investigation of Cryptosporidium (Tyzzer, 1907) species with molecular methods in calves in around of Kutahya province

The protozoan parasite Cryptosporidium spp. is causing Cryptosporidiosis and this disease is getting increasingly importance. These protozoan parasites which are found to be at intracelullar-extracytoplasmic area in intestinal cells, are known to cause a zoonotic infection. It has a large host potential, including wild animals, especially human beings and pets. Cryptosporidium parvum species is the most known zoonotic species of this disease. In our study, 100 stool specimens were collected from calf groups age ranging from 1 day to 4 months in around of Kutahya province and molecular characterization of these samples was done by using PCR-Nested PCR and RFLP methods. Cryptosporidium parvum, Cryptosporidium bovis and Cryptosporidium ryanae are the species found in calves in the region of Kütahya, respectively. These identified species were found to be in same cluster with predetermined strains in many parts of the world primarly in China and also in Turkey as a result of phylogenetic analysis of positive isolates by using the Kimura-2 Parameter method.