Investigation of co-immobilization of lipase and peroxidase and their use for treatment of industrial effluent

Objective: This research was carried out to investigate co-immobilization of horseradish peroxidase (HRP) and Aspergillus niger lipase enzymes onto non-magnetic and magnetic natural polymers and their use in the treatment of industrial wastewater. Material and Methods: Co-immobilization was performed on alginate and chitosan based magnetic and non-magnetic beads. Optimum pH, optimum temperature and kinetic parameters of free and immobilized enzymes were determined. Thermal, pH and storage stability studies were carried out. Decolorization of synthetic dye samples using immobilized HRP enzyme was investigated. Removal of phenolic compounds from olive oil mill wastewater for industrial application and the degradation of olive oil to fatty acids through lipase hydrolysis were investigated. In addition, reusability tests were carried out in olive oil mill wastewater. Results: It was observed that there were no significant differences in the optimum pH, optimum temperature and kinetic parameters of free and co-immobilized enzymes. It has been determined that each enzymes catalyze its specific reaction in the vicinity of the other enzyme. İmmobilized enzymes weremore resistant to environmental conditions such as storage duration, temperature and pH compared to free enzymes. It was measured that with free and co-immobilized HRP enzymes decolorization of synthetic dye samples was 40-60%; the phenolic compound removal in olive black water was in the range of 60-75%. The degradation of olive oil into fatty acids by lipase hydrolysis was successful in olive oil mill wastewater. Conclusion: In this study, co-immobilization of HRP and A. niger lipase enzymes onto natural polymer was performed successfully; decolorization, total phenolic compound removal and the use of olive oil in the degradation of fatty acids were investigated. Keywords: HRP, Lipase, Co-immobilization, Wastewater Treatment, Olive Mill Wastewater.