Investigation of catechol-o-methyltransferase (COMT) Val158Met polymorphism in chronic schizophrenia diagnosed individuals in mani̇sa

The study was conducted with 100 healthy individuals who were diagnosed with chronic schizophrenia and 100 healthy controls that did not have any psychiatric disorders in their family. The patient group was individual; Manisa Psychiatric Diseases It is composed of individuals who are treated in the hospital and diagnosed with chronic schizophrenia. For each individual in the study group DNA isolation was followed by high or low activity alleles associated with the Val158Met polymorphism in the COMT gene using the PCR-RFLP method. Data were obtained using Chi-Square Test and Crosstab. Findings: 64% of individuals surveyed were female, 36% male. When the genotypes of the patients were evaluated without regard to sex, 44% of the subjects were from LL genotypes, 10% were from HH genotypes and 46% were from HL genotypes.When allele frequencies calculated output in Hardy Weinberg Equation. For the patient group, the Val allele (high activity) frequency was 33%, the Met allele (low activity) frequency was 67%; The control group had 67% frequency of the Val allele and 33% of the Met allele frequency. Results: According to the genotype findings, the cross-tabulation and Chi-square test results were found to be the effect of COMT Gene Val158Met polymorphism on schizophrenia when p = 0.001 for HH and LL alleles. Keywords: Catechol-O-Methyltransferase (COMT), PCR-RFLP Val158Met Polymorphism.