Markır yardımlı geriye melezlme ile fusarıum solgunluğuna (Fusarıum oxysporum Schlecht. f. Sp. melongenae) karşı dayanıklı patlıcan geliştirilmesi

Eggplants are produced in both greenhouse and open field, and fungal diseases cause significant yield loss. Fusarium oxysporum Schlecht. f. sp. melongenae, FOM) is a major soil-borne pathogen, causing vascular wilt disease in eggplant. A molecular marker tightly linked to single dominant gene (FOM) was previously available for use in marker assisted selection (MAS). The aim of the study was to develop eggplant lines resistant to Fusarium wilt by using a marker assisted backcross breeding approach. Donor parents were advanced eggplant lines known to have fusarium wilt resistance originating from "LS2436" (Solanum melongenae) lines. The eggplant breeding materials was first screened with the molecular markers linked to the FOM gene. Then, the 533 young seedlings representing various populations claimed to be resistant to the pathogen were both root-dip inoculated with FOM isolate obtained from BATEM institute (Antalya, Turkey), and screened with the molecular marker. All the seedlings identified as resistant using the markers survived the infection. Marker assisted selection and backcross programme was continued to BC1F1. The seedlings of BC1F1 population along with the parents and checks were again screened with molecular marker and then classical test. Results showed that all the plants selected by MAS showed resistance response to FOM in classical test. Resistance vs susceptible ratio was 1:1 as expected in BC1F1 generation. In conclusion, the marker is reliable for selection against FOM and developing new eggplant cultivars resistant to FOM via marker assisted backcross selection is feasible.