The detection and characterization of Lettuce big-vein disease and Lettuce mosaic virus

This study was conducted for detection of and to investigate of causative agents causing Lettuce big-vein disease (LBVD) and diagnosis and characterization of Lettuce mosaic virus (LMV) in the fields where the lettuce cultivation was made extensively in Adana and Mersin provinces in autumn and winter of 2015-2017. A total of 160 lettuce plants suspected to be infected with LBVD (MiLBVV, LBVaV) or LMV were sampled at the field simptomologically. As the result of ELISA tests, MiLBVV and LMV were detected in 53 and 22 plants, respectively. In the RT-PCR studies for MiLBVV, the band a size of 233 bp was obtained by MiLBVV(1)-MiLBVV(2) primer pair for isolates collected from Cukurova, Seyhan, Karaisalı, Yakapınar and Yenice regions; the band a size of 296 bp was obtained for Cukurova, Karaisalı and Yenice isolates by LBVaV specific VP-248 and VP-249 primer pair; a band of 360 bp was observed for isolates collected from Yakapınar, Yumurtalık and Yenice by LMV specific LMV-CPR; LMV-CPF primer pair. As a result of sequence analysis, while Yenice-1 and Yenice-2 isolates of MiLBVV were in the same group, Adana and Mersin isolates of MiLBVV were in different groups. While Yüreğir isolate of LBVaV in the same group with Saudi Arabia isolate, the Yenice-2 isolate used for LMV did not take part any other country isolate.