The investigation of the effects of apelin on mcf?7 human breast cancer cell line: an in vitro study

SUMMARYApelin is the endogenous ligand for the G-protein-coupled APJ receptor. That Apelin has been found in hypothalamic fields such as the periventricular nucleus and supraoptic nucleus of the hypothalamus and that it is seen in testicle, prostate and mammary cells suggest that apelin may be influential on productive system. Apelin varies depending on the forms on which it is influential. As the sequence of 13 amino acids in apelin can not be changed, the basic apelin structure is accepted as apelin-13.In the study, firstly, 0.1, 1 and 10 ?M concentrations of Apelin?13 and 1, 10, 100 ?M concentrations of estrogen are applied into the line of estrogen sensitive breast cancer cell ( MCF?7). Apellin 0.1, 1 and 10 ?M and estrogen hormone 1, 10, 100 ?M were simultaneously cultured and incubated for 24 hours and their effectiveness were detected by means of 3-(4,5-dimethylthiazol?2-yl)-2,5-diphenyltetrazolium bromide (MTT).It has been detected that Apelin?13 applied into MCF?7 line decreases the cell liveliness, and estrogen increases cell liveliness. (p<0.05)These results are estrogen receptor positive MCF-7 cells, is induced suggests that proliferative effects of estrogen receptor.Key words: Apelin, MCF?7, Cancer, MTT assay, Cell culture