Assesment of adhesion, viability and osteogenically differentiation capacity of human adipose tissue derived mesenchymal stem cells which is grown on metal ion (zn, ag and cu) doped nanohydoxyapatit coated surfaces, by in vitro methods

In this study, human adipose tissue derived mesenchymal stem cells (MSC)', that grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coating surfaces which have different chemical and surface properties, viability, osteogenic differentiation capacity and interactions between substrate-material and cell behavior, were investigated. For this purpose, viability of the cells on biomaterials were determined by MTT, LDH and DNA quantitation methods. The osteogenic differentiation capacity of MSCs which is grown on biomaterials were analyzed with Alizarin Red (AR) Staining, biochemically by Alkaline Phosphatase Specific Activity (ALP) and Osteopontin gene expression level by rt-PCR. Furthermore, adhesion of the MSCs on biomaterials and the effect of cell adhesion on biomaterials biocompatibility were detected by cell adhesion assay, immunofluorescence staining for vinculin and f-actin cytoskeleton component and rt-PCR array which including 84 extracellular matrix proteins and cell adhesion pathway genes. The results generally demonstrates that viability of MSCs, osteogenic differentiation of the cells in without osteogenic stimulation in medium and cell adhesion capability are higher on nano-coating surfaces that including Zn, Ag and Cu metal ions than commercial HAP. This results reveal that these metal ions contribute to biocompatibility of material.