Obtaining metal nanoparticle doped reduced graphene oxide nanocomposites using green synthesis, characterization, determination of their cytotoxic, antioxidant and electrochemical potentials

In thesis study we synthesized reduced graphene oxide and reduced graphene oxide metal nanocomposite via by parasitic Orobanchae aegyptiaca Perss. at first time. The obtained nanostructures characterizated using UV-Vis spectroscopy, X- Ray diffractiometer (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM) and X-Ray photoelectron spectroscopy (XPS). UV-Vis spectroscopy results confirm that graphene oxide and metal salts are reduced simultaneously by the phytochemicals contained in the O. aegpytiaca extract. The morphologies of the graphene based materials were examined by SEM and TEM analysis and it was observed that the metal nanoparticles were distributed homogeneously on the surface of the reduced graphene oxide. Cytotoxic antioxidant and electrochemical potentials of the structures obtained within the aim of the thesis study were determined. The synthesized graphene based materials has showed different effects in human lymphocyte cells. It has been observed that rGO and rGO-Pd nanocomposite structure has a lower cell life groups than other synthesized substances. In this study, the antioxidant activity of synthesized graphene based structures was determined by performing various in vitro tests. As a result of antioxidant tests, it was determined that the synthesized graphene based materials obtained had moderate antioxidant potential. In this study that carbon paste electrodes (CPE) were prepared with the obtained rGO and metal-doped rGO nanocomposites, and their electrochemical characterizations were examined by cyclic voltammetry (CV) technique. Then, the prepared modified KPEs were used to examine the electrochemical behavior of metronidazole and it was observed that the prepared KPEs responded well to the electrochemical reduction of metronidazole.