The role of MGP gene polymorphism in coronary artery disease

Coronary artery calsification (CAC) is associated with coronary atherosclerosis. There is a significance relation between coronary artery wall calsification and coronary artery disease(CAD). The measure of coronary artery calsification is an indicator of subclinical atherosclerosis . İn some studies it has been showed that independent from convantional cardiovascular risks , coronary artery calsification suggests data about prognosis. Matrix Gla protein (MGP) appears to be an important protective modulator against calsification since it is a potential inhibitor of tissue calcification. Therefore , MGP as an inhibitor of calsification recently has been more important in studies.In this respect, it is aimed to establish the relationship between the distributions of nucleotide alterations found in promoter and coding regions of MGP gene in patients with coronary artery disease (CAD) and patients that have not coronary artery disease.DNA samples were obtained from 58 patients with coronary artery disease (CAD) and 57 patients that had not CAD (Totally from 115 patients). The DNA samples obtained were analyzed by Polymerase Chain Reaction (PCR) method using the 3 sets of primer pairs which cover the coding (Thr83Ala in exon 4) and promoter regions (T-138C and G-7A) of MGP gene. Amplified regions were analyzed by Restriction Fragment Length Polymorphism (RFLP) method for possible polymorphisms.The chi-square analysis of the results revealed that there is no relation between the observed polymorphisms and CAD.Keywords: Coronary Artery Disease, Coronary Calsification, Matrix Gla Protein, MGP Gene Polymorphism