Molecular pathology between helicobacter pylori (+), helicobacter pylori (-) intestinal metaplasia and gastric adenocarcinoma

Molecular Pathology Between Hp(+), Hp (-) Intestinal Metaplasia And Gastric Adenocarcinoma Introduction and Aim: Intestinal metaplasia (IM), is an important risk factor for development of gastric cancer. WNT signalling pathway plays an important role in carcinogenesis. In our study, we aimed to evaluate the diagnostic and prognostic value of the genes in WNT signalling pathway in gastric cancer patients. Method: A total of 104 patients, 34 patients with gastric cancer, 45 patients with intestinal metaplasia and 25 healthy controls, were included in this study. expression of genes in WNT signalling pathway were evaluated and compared in Gastric cancer, IM and control groups by RT-PCR array method. gastric cancer patients were divided into groups according to presence of metastasis and localization of the cancer in stomach. Intestinal metaplasia patients were divided into groups according to presence of Helicobacter, which is accepted as a risk factor for gastric cancer. Result: RHOA, CSNK1A1, DVL2, FZD8 and LRP5 were overexpressed in gastric cancer and IM groups, compared to control group. RHOA, CSNK1A1, DVL2, FZD8 and LRP5 were significantly overexpressed in patients with metastasis compared to patients without metastasis. RHOA, CSNK1A1, DVL2, FZD8 and LRP5 were significantly overexpressed in patients with diffuse gastric cancer compared to patients with non-diffuse gastric cancer. there was no significant difference in gene expression between gastric cancer group and IM group. RHOA, CSNK1A1, DVL2, FZD8 and LRP5 were significantly overexpressed in Hp + patients compared to Hp- patients. Conclusion: We think that RHOA, CSNK1A1, DVL2, FZD8 and LRP5 reflect tumour burden in patients with gastric cancer. Thus they can be used as biomarkers in the future while monitoring treatment. We think that ratio of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 expressions begin to increase before gastric cancer, and presence of Hp increases the expression. Further studies are needed. Key Words: Gastric Cancer, Intestinal metaplasia, RHOA, CXADR, CSNK1A1, CCND2, DVL2, FZD8, NFACT1