New molecular recognition technologies for mimicking the active sites of enzymes on micro and nano surfaces

Molecular recognition is a crucial phenomena for living organisms. This study is aimed to mimic the active site of lipase on the micro and nano surfaces by using new molecular recognition techologies. For this purpose, firstly, functional monomers methacryloylamido histidine (MAH), methacryloylamido serine (MASe) and methacryloylamido glutamic acid (MAGA) were synthesized and then p-nitrophenyl palmitate microbeads were prepared by suspension polymerization technique. In the second step, surface of the iron nanoparticles was coated with p-nitrophenyl palmitate imprinted polymeric shell. In the third step, polymer-clay nanocomposite materials having p-nitrophenly palmitate imprinted polymer between their nanolayers were prepared. The characterization of the prepared enzyme mimic structures was carried out by swelling tests, BET, FT-IR, SEM and TEM. In the last step of the study, the efficiencies of the prepared micro and nanostructures for the p-nitrophenyl palmitate hydrolysis, biodiesel production, jasmonic acid and oleic acid esterifications were investigated.