Mikroalg yetiştirme ve mikroalg biyokütlesinden biyo-yağ üretimi

In this research, production of bio-Oil as a renewable biofuel from microalgae biomass was studied. The work was divided into four main steps. Firstly, wild-type microalgae species from local habitat were isolated, identified and characterized based on their potential for biofuel production and/or carbon dioxide fixation capacity. The highest measured lipid content was 47.32 % for Scenedesmus quadricauda (I). The attempt for improving lipid extraction efficiency from microalgae cell was the subject of second step. The effect of adding microparticles of glass as a cell disruption enhancer to maximize the extracted lipid from microalgae was investigated. With the addition of 40 µm particles in 2.25 mass proportion of particle to dried biomass, lipid extraction from Chlorella sp. enhanced by 46.60 %. In the third step, the aim was to maximize the growth rate of the selected microalgae Scenedesmus sp. in the first step which was the most proper one for bio-oil production. With the optimized values of 6.3 % CO2, 1.03 vvm flow rate, 1.47 mm diffuser diameter and 0.230 g/l initial inoculation, the maximum growth rate of 0.513 g/l.d was estimated. At the forth step, biomasses of microalgae Scenedesmus and Synechosystis species were thermochemically converted to biofuel in a fast pyrolysis process. The best pyrolysis temperature for Scenedesmus and Synechosystis biomass was respectively 500 oC and 600 oC resulted in higher biooil yield of 80.0 and 71.0 wt %.