Comparison of PDGF-BB and il-6 levels in gingival crevicular fluid after use of titanium-prepared platelet rich fibrin and connective tissue grafts in the treatment of miller class i and ii defects

Aim: In this study, it was aimed to comparatively investigate the clinical and biochemical effects of subepithelial connective tissue graft and coronally advanced flap (SCTG + CAF) or titanium-prepared platelet rich fibrin and coronally advanced flap (T-PRF + CAF ) in the treatment of Miller class I and II gingival recessions. Material and Method: Twenty-two patients with Miller I and II gingival recession were included in our study. Individuals were randomly assigned to the test and control groups. Thirty four defects were treated with T-PRF + CAF (test) or SCTG + CAF (control) technique. Gingival index (GI), plaque index (PI), keratinized tissue width (KTW), gingival recession depth, gingival recession width and mean root surface coverage were measured at baseline, 1st, 3rd and 6th months. Probing depth (PD), clinical attachment level (CAL) and gingival thickness (GT) were assessed at baseline and 6 months. Gingival Crevicular Fluid (GCF) samples were collected from patients before periodontal surgery and after 1st, 3rd and 6th months, PDGF-BB and IL-6 values were determined by ELISA method and evaluated statistically. Results: After 6 moths, mean root coverage was 69,21% and 76,27% respectively, complete root coverage was 47,05% and 64,7% respectively, at test and control sites. At 6 moths after surgery, there was no statistically significant difference between groups in clinical data, except for greater gain in gingival thickness obtained in CTG group. Gingival crevicular fluid levels were significantly elevated in both group at 1 month compared with baseline, but significantly decreased in follow-up periods. In the test group, IL-6 level was significantly decreased after 1 month of operation compared to baseline, no difference was observed in the control group. There were no statistically significant differences in PDGF-BB levels between follow-up periods according to baseline in both groups. No significant differences were found in biochemical parameters at 1, 3, and 6 month between study groups Conclusion: The data in our study have shown that T-PRF membranes provide reliable and effective results in the treatment of Miller I and II gingival recessions without the related to morbidity or difficulties associated with a second surgical site. At 1 month, the reduced gingival crevicular fluid levels of IL-6 may support the anti-inflammatory effect of T-PRF on wound healing. T-PRF can be applied as an important alternative to CTG.