Molecular determination of the ure a gene in Klebsiella pneumonia with hypermucoviscos resistant to multipleadents isolated from hospital patients

Hypermucoviscous Klebsiella pneumoniae is an emerging pathogen. In this study two hundred clinical samples were collected randomly from patients suffering from urinary tract infections. The collected samples were cultured on selective and differential media. The isolated bacteria were identified. Antibiotic susceptibility was detected by vitek system to detect the multidrug resistant bacterial isolates. String test was adopted to confirm the identification of hypermucoviscous type. Urease production by Hypermucoviscous Klebsiella pneumoniae was characterized phenotypically by Christensen medium. Ure A gene was detected using selective primers designated for this study. Polymerase chain reaction was adopted to detect the presence of urease coding gene. The resuls revealed that there were differences between the genetic and phenotypic methods to detect urease. PCR was more accurate as it detected the ure A gene in all the isolates (100%) while the phenotypic method results revealed that the positive urease occupied 70.5% of all the isolates.