Molecular and pathological study of proteus Spp. isolated from uti infected diabetic patients in Salah aldeen province in Iraq

This study concentrated on the isolation and identification of Proteus mirabilis isolates from diabetic mellitus patients with urinary tract infections. 100 clinical specimens of mid-stream urine were collected from male and female Iraqi patients who visited Salah al-Din general hospital. On the other hand, all urine specimens from patients were cultured on blood agar and MacConkey agar. The results of the samples were distributed as 71% and 29% of females and males, respectively. The results of the current study showed that the highest rate of UTIs was for the age group between 30 and 40 years. According to the cultural, morphological, biochemical, and vitek, only 23 isolates (23%) were identified as Proteus mirabilis. DNA was extracted from bacterial isolates to detect 16s RNA, hmpA, and ureC genes. PCR technique was used for detection these genes via specific primers. The results of the current work recorded (100%) for 16sRNA, hmpA, and ureC genes. On the other hand, the highest rate of antibiotics sensitivity for all isolates were amikacin and ciprofloxacin (73.91%). In addition, the highest rate of antibiotics resistance were Ampicillin and Norfloxacin (95.65%). The result of the sequencing test for 16s RNA showed the genetic affinity and divergence between Proteus mirabilis isolates.