Relationship between neuroblastoma and microRNA

Introduction: Neuroblastoma tumors, presents % 8-10 of all childhood cancers. MicroRNAs (miRNA) are small and noncoding RNAs which play important roles at regulation of posttranscriptional gene expression and pathogenesis of some cancers. The aim of this study was to determine the miRNA expression profiles of neuroblastomas with different clinical and histological characteristics. Material and methods: In this study 24 samples from 17 patients paraffin blocks, were used. Tissues were seperated into groups and their microRNA profiles were compared by 5 different analysis: Analysis I: Well (n=6), poorly (n=6) differantiated Analysis II: Before (n=3), after (n=3) chemotherapy Analysis III: Favorable (n=10), unfavorable (n=8) histology Analysis IV: Neuroblastoma (n=4), ganglioneuroma (n=4) Analysis V: Low (n=10), middle (n=6), high (n=8) risk After bioinformatics analysis Real-time PCR was performed for validation. Validated microRNAs were compared of high- and low-risk new patients' tumors.‬ Clinical data were compared with differentially expressed microRNAs. Results: 25 miRNA in analysis I, 8 miRNA in analysis II, 3 miRNA in analysis III, 4 miRNA in analysis IV, 7 miRNA at low-middle risk, 1 miRNA at middle-high risk, 14 miRNA at low-high risk comparison in analysis V were found different (p < 0.01). These miRNA's targeted mostly the cancer pathway by the KEGG pathway analysis. ‬The markedly difference were at miR-132 and miR-490, comparing the clinical data and microRNAs. The most fold change was detected at miR-98-5p between the tissues of high- and low-risk patients. Conclusion: Our study can be informative for therapies which targets microRNAs and for biomarker studies. ‬ Keywords: microRNA, neuroblastoma, ganglioneuroma