Onosma mutabilis (O. mutabilis) bitki özütünden sentezlenen gümüş nanopartiküllerin antioksidan ve antidiyabetik aktivitelerinin araştırılması

ABSTRACT EVALUATİON OF THE ANTİOXİDANT, ANTİDİABETİC OF SİLVER NANOPARTİCLES SYNTHESİZED USİNG ONOSMA MUTABİLİS (O. MUTABİLİS) ROOT EXTRACT This study aimed to investigate the antioxidant and antidiabetic effects of silver nanoparticles synthesized by the green method using the root extract of O. mutabilis plant. In this context, the roots of the plant were extracted by maceration method using water and the green synthesis of silver nanoparticles (AgNP) was carried out and characterized under different conditions with the obtained plant extract. AgNPs were characterized by UV-Visible Field Spectroscopy (UV-Vis), X-Ray Diffraction (XRD), Particle Size Measurement (Malvern Mastersizer), Scanning Electron Microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FT-IR). As a result of the characterization, it was determined that the nanoparticles were approximately 26-100 nm in size and cubic in size, showing a spectrum at 410 nm wavelength. The antioxidant activity of the extracts was investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH-) free radical scavenging activity method. The results obtained were evaluated by comparing with Butyl hydroxytoluene (BHT) used as a standard substance. For the determination of total phenolic matter (TFM), Folin-Ciocalteu method was used and the highest amount of phenolic matter in the extracts was determined as 425±0.07 mg/g as gallic acid equivalent. For antioxidant activity, the highest DPPH free radical scavenging activity % inhibition value was 59.36. The antidiabetic activity was determined by measuring alpha-amylase inhibition by spectrophotometric method. The alpha-amylase inhibition values of the sample showed the highest 35% inhibition ability. In conclusion, it can be concluded that silver nanoparticles synthesized from O.mutabilis plant may enhance the antioxidant status with their significant phenolic component content and also prevent postprandial hyperglycemia as a nutraceutical due to their partial α-amylase inhibition activity. Keywords: Onosma Mutabilis, Green Synthesis, Silver Nanoparticles, Phenolic Compound, Antioxidant Activity, Anti-diabetic Activity. Advisor: Asst. Prof. Pelin EROĞLU, Department of Chemistry, University of Mersin, Mersin.