Comparison of clinical sample collection methods and different isolation media results in diagnosis of Pityriasis versicolor

Pityriasis versicolor (PV,tinea versicolor) is a superficial fungal infection of the skin, appearing most commonly on the upper trunk, as well as on the upper arms, neck and face. PV may causes in different colours, ranging from pink or tan to dark brown or even black. The lesions have a characteristic flaking appearance, although in larger lesions this can be evident only at the border of the macule, and can be hypopigmented or hyperpigmented. Generally, the lesions take the form of round or oval macules or papules, although in advanced cases the lesions may become confluent. Whereas some patients experience mild pruritus, PV is generally asymptomatic, and most patients complain primarily about the cosmetic appearance of the diseaseIn the study, diagnosed that 57 PV patient included. The diagnosis of PV that sampling from PV lesions was established by the characteristic ?spaghetti and meatballs? appearance. Samples from with a sterile scrapel and cellotape inoculated on modifiye-Dixon and Leeming- Notman agar. Culture were incubated at 32 °C and were waited for 5 days. After growth, catalase test was tested. Growth in Chromophor-EL, Sabouraud glicose agar was evaluted. Pigment on Chrom Candida Agar(CCA) was examined. As identification of Malassezia was used Tween test which prespitating Tween 20, 40, 60, 80 was determined. To examine blastospor morphology, lactophenol cotton blue and Gram stain was done.In the study, samples from lesions that was examined with KOH direct microscopy were showed ?spaghetti and meatball? appearance. All of isolates were catalase pozitive. Six of samples did not grow on both medium. Seven of samples were not determinated because of the fact that they could?t isolated more. Other samples that were isolated well, were applied Tween asimilation test and diagnose tests. 27 of samples were M. furfur, 10 of samples were M. slooffiae, four of them M. sympodialis, two of them M. globosa and another two of them were not compatible with Tween compoundWhen comparing collection of samples, scraping method were more beter then cellotape band and when using scraping method collection of dandruff were much more than other. Eventuality much contain of microorganisms were seen. Cellotape method was seen unusefull. As performing media, coloni on modifiye-Dixon media grew much more and very bigger than Leeming- Notman media. In the study, ın addition Chremophor-EL and Tween 60-eskülin test were not compatible with species identificationWhen results of survery were evaluated, PV was more commen men. As an age it was commenly seen between 20-30 age. Another results of the study was that PV was more recurren and the lesions in both sex were observed commonly on neck, back and chest. Correlation wood light and pigmentation between species was not associated with.