Expression of the gene coding enzyme lactate dehydrogenase from Plasmodium vivax purification of the related protein and its analysis

ABSTRACTMASTER THESISEXPRESSION OF THE GENE CODING ENZYME LACTATE DEHYDROGENASEFROM PLASMODIUM VIVAX PURIFICATION OF THE RELATED PROTEIN ANDITS ANALYSISAbdullah ASLANFırat UniversityGraduate School of Natural and Applied SciencesDepartment of Biology2006, Page : 50Increasing resistance of Plasmodium against currently available antimalarials necessitatesdevelopment of new and effective drugs. In this thesis; gene encoding lactate dehydrogenasefrom Plasmodium vivax was amplified by adding 6 histidines to the C-terminal of the enzymeprior to expressıon of the protein in the expressıon vector pKK223-3, in E. Coli. Theoverproduced protein was then purified by using Ni-NTA Spin kit and Ni-NTA agarose andanalysed kinetically. An antibody produced against PfLDH was tested against this kineticallyanalysed pure PvLDH protein. It was shown that two proteins were similar enough that PvLDHreacted with PfLDH antibody. It is expected that the drug design studies applied on PfLDH arealso made applicable on PvLDH after obtaining pure PvLDH protein. As a result it is expectedthat, if we develop one antimalarial against one at the Plasmodium species would work againstother species too.Keywords: Malaria, Plasmodium vivax, Lactate dehydrogenase, Expression, Purification.