Proliferation and differantion effect on human mesenchymal stem cells by using prolactin hormone

One of the most important issues in stem cell applications is the transfer of pure cells to the damaged tissue in sufficient number and quality to repair tissue. Therefore, biological stimuli such as recombinant or synthetic cytokines, growth factors, play a key role in the differentiation and proliferation of undifferentiated stem cells into mature cells. In this study, Mesenchymal Stem Cells were obtained from the placenta provided under sterile conditions in a laminar cabinet. These MSCs were planted in T75 flasks and cultured for 14 days at 37 0C in a CO2 incubator. At the end of this period, trypsinization was performed (Passage 0). After passage 0 (P0), the samples were re-seeded and trypsinized on Day 7 (Passage 1). The study was performed with the cells obtained in P1. To determine the dose of prolactin hormone, starting from 102 ng / L to 96 wells, decreasing doses (50, 25, 10, 1 10-1 10-2 10-3 10-4 10-5 ng / L) were controlled for each dose. wells were seeded with a standard number of cells. At the same time, 8 wells were cultured with medium (medium) for negative control. MTT (tetrazolium salt 3- [4,5-dimethythiazol-2-yl] -2,5-diphenyltetrazolium bromide), a commonly used enzymatic test, was performed to determine the most effective prolactin dose on cell culture. When the results were evaluated, no significant statistical difference was observed between the doses. In order to determine the effect of prolactin hormone on MSC osteogenic and adipogenic transformation, 20 ng / L prolactin dose was cultured. Cell cultures were made using only 20 ng / L prolactin dose with hormone, hormone and adipogenic transformation medium and hormone and osteogenic transformation medium. Culture was monitored in the CO2 incubator and the effect of this hormone on differentiation tested. Oil Red-O staining protocols were used to test adipogenic transformation and Alizarin Red S staining protocols were applied to test osteogenic transformation and the result was evaluated microscopically. According to the results, a similar number of transformed cells were observed in the cultures using prolactin hormone.