A comparative investigation of the effect of application propolis and hypericum perforatum as A cavity disinfectant on the physical properties of resin based fissure sealent

It is aimed to prevent the development of caries and stop the initial lesions in children and adolescents with pit and fissure sealants commonly used within the scope of preventive therapies. Before applying the fissure sealent, the plaque should be removed from the tooth surface. For this purpose, some different surface cleaning techniques are applied. However, ıts very well known that residual bacteria may still exist in pits and fissures. It is appropriate to use a cavity disinfectant to eliminate bacteria remaining after mechanical cleaning in pits and fissures. This study aims to compare the effects of the use of propolis and hypericum perforatum extract as cavity disinfectant on the physical properties of the fissure sealant material prior to the application of the fissure sealant material. In our study, the shear bond strength and microleakage were investigated using human third molar teeth under in vitro conditions. The method of dye penetration has been chosen to evaluate the microleakage and the method of shear test method has been chosen to evaluate bond strength. Negative control group samples were not disinfected. Disinfection with chlorhexidine was applied to the positive control group, disinfection with propolis and disinfection with hypericum perforatum were applied to the experimental group samples before fissure sealant. For shear bond strength test, the fissure sealant material was applied with the plastic tubes prepared 3 mm diameter and 2 mm height. The force is applied to the specimens at a constant speed of 1 mm / min until rupture occurs. Broken sample surfaces examined under stereomicroscope are grouped as adhesive, cohesive, mixed type. Groups used for shear bond strength testing were also created for microleakage testing. Fissure sealant was applied to the occlusal surfaces of third molar teeth. After 500 cycles of thermal cycling, the teeth were kept in 0.5% methylene blue solution for 24 hours, and three sections were taken from the specimens in the bukkolingual direction. Sections were examined at X40 magnification under stereomicroscope. The application of propolis and hypericum perforatum before the fissure sealant did not affect the shear bond strength in samples showing the adhesive and cohesive refraction type. The mean MPa value of the samples showing the mix fracture type showed a significant difference (p<0.05). The application of propolis increased shear bond strength in samples showing mixing fracture type compared to the negative control group. The application of hypericum perforatum increased the shear bond strength in the samples showing the mixing fracture type compared to the negative control group and the positive control group. According to the results of microleakage, before the fissure sealent there was no significant difference between the control groups and experimental groups applied propolis and hypericum perforatum (p> 0.05). The use of Propolis and Hypericum Perforatum extract for disinfection did not affect the physical properties of resin based fissure sealant. It has been concluded that these natural substances with antimicrobial properties can be used as cavity disinfectants. However, we need further studies to be used and standardized in the field of dentistry. Key words: Bond strength, propolis, hypericum perforatum, microleage, pit and fissure sealent