Development of the radioprotector biopolymer systems

It has been found that some chemical substances, which is taken in organism before exposure to ionizing radiation, protect the organism against lethal and harmful effect of ionizing radiation called as ?radioprotector?. They protect the organisms against a lethal dose of ionizing radiations when they are also extremely small amount. Various compunds have been investigated towards on radioprotective effect but polymer containing have not been discovered up to now. Since the organic compounds investigated radioprotective effect have relatively less molecular weight, so that they show biological effect in a short time. That is why it is very important to improve polymeric radioprotector. In the present work, ion-coordination compounds of some water soluble negatively charged polyacrylic acid (PAA) and copolymer of acrylic acid vinylpyrrolidone (P(AA/VP), neutral polyvinylpyrrolidone (PVP) mediated with Cu(II) and Bovine Serum Albumin (BSA) which is found the highest protein fraction in blood have been used. Chemical methods (uv-vis, FT-IR spectrophotometry, HPLC, elemental analysis, AAS) have been employed to these complexes in order to investigate the effect of irradiation. The effect of radiation on in vitro human peripheral lymphocyte in the presence of these compouds have been researched using cytogenetic methods (micronucleus analysis (MN), chromosome aberration (CA) analysis). My work has been focused on polymers having several different molecular weights and conformations in order to understand the biological effect mechanism. Until certain radiation doses, reduction of Cu(II) ion to Cu(I)ion in polymer-metal complex system by rising radiation effect has increased. These results have confirmed, the radiation energy was absorbed by Cu(II) ion in polymer-metal complex system. It has been showed that in cytogenetic studies, the effect of molecular and conformational alterations were difference on cell. The role of interpolymer complex formation and cooperative interaction have been pointed out in the interaction of PAA and their complexes with cell. It has been determined that, in specific PAA concentration has radioprotective effect. The objective of this study is to develop high-effective polymer containing radioprotectors with low toxicity as an alternative to the conventional radioprotectors mentioned before. Keywords: Radioprotector, water soluble polymer, PAA, polymer-metal complex, MN, CA.