Investigation of electrophysiologic effects of OMAGA 3 fat after acute trauma in rats

Acoustic trauma is a common reason for hearing loss and it can result with permanent or temporary hearing loss due to hair cell degeneration. since Omega-3 fat is a well known antioxidant for cell protection and strengthening immune system, the aim of this study is to evaluate the usage of Omega 3 on acoustic trauma 24 healthy, mean weight 350 gr and mean age of 12 months of Sprague Downey male rats were included in our experimental study. Rats were examined in 3 equal number of groups. 1st group was given Omega-3 fat pre and post acoustic trauma (150mg/kg). 2nd group was not exposed to acoustic trauma , only given Omega-3 fats, and 3rd group was exposed to only acoustic trauma and not given Omega-3 fats. DPOAE test were done 3 times to each group of rats as; before acoustic trauma, after acoustic trauma and 10 days later of acoustic trauma. Prior to the acoustics trauma, hearing threshold results of the rats were similar (p> 0,005) .when two groups that were exposed to acoustic trauma were compared; 1st group of rats that were given Omega-3 before acoustic trauma was examined that omega-3 has a preservative effect on acoustic trauma due to similar DPOAE thresholds before and after. The group of rats which were exposed to acoustic trauma without any omega-3 given had a significant decrease of DPOAE thresholds after acoustic trauma, but able to recover almost the first thresholds after 10th day of the exposure. The group of rats that were given only Omega-3had no negative or toxic affect of Omega-3 usage. As a result , in comparison of groups; preservative affect of usage of Omega-3 fats was determined due to acoustic trauma exposure , However, further studies concerning on dose and acoustic trauma model should be examined. Key Words: Acoustic Trauma, Omega-3, Hearing, DPOAE