Investigation of the effect that iloprost and n-acetylstystine on bowel ischemia-reperfusion model on ischemia-reperfusion damage and the thyol / disulphite balance

SUMMARY INTRODUCTION AND PURPOSE: Intestinal ischemia occurs after partial or complete obstruction of the intestinal arterial blood flow and reperfusion injury occurs with the re-supply of blood flow. Intestinal Ischemia Reperfusion (IRR) damage can cause multiple organ failure and death. The aim of this study is to investigate the effect of Iloprost (IL) and N- acetylcysteine (NAC) on IRR damage. MATERIALS AND METHODS: 30 Sprague Dowley rats weighing between 200-300 g were divided into 5 groups, each with 6 subjects. The rat was shaved in a supine position by giving ketamine xylazine, and the rats were stained with povidone iodide and entered into the abdomen by making a median incision. Only intestinal samples and blood were collected from the Sham group without any surgical procedures. In IIR group, KMA was dissected and intestinal ischemia was performed with a clamp. The skin was closed with skin staples. When the 45-minute ischemia time was completed, the abdomen was opened. KMA declamped and the skin was closed with skin staples to allow for 120 minutes of reperfusion. After reperfusion the abdomen was reopened and animals were sacrificed and intestinal samples and blood were collected. In IIR + IL group, 2mcg / kg Iloprost I.P was given half an hour before reperfusion and the same procedures were applied. In IIR + NAC group, N-Acetylcysteine was given as 300 mg / kg I.P half an hour before reperfusion and the same procedures were performed. In IIR + NAC + IL group, the same procedures were applied by giving 2mcg / kg Iloprost I.P and N-Acetylcysteine 300 mg / kg I.P, half an hour before reperfusion. RESULTS: The average of Chiu Score of the IIR + NAC group was lower than the IIR group and the IIR + IL group, but there was no statistically significant difference between these groups(p>0,05). Sham group scores were determined to be significantly lower and statistically significant compared to IIR, IIR + IL, IIR + NAC, IIR + NAC + IL scores(p<0,05). Reduced Thiol, NT / TT ratios were increased in the groups where NAC, IL and the two were given together, compared to the IIR group, and Oxidized Thiol, Disulfide / TT and Disulfide / NT ratios were decreased compared to the IIR groups(p<0,05). When the groups given the drug were compared with each other, the Reduced Thiol, NT / TT ratios of the group given NAC were higher than those of IIR + IL and IIR + NAC+IL groups, but there was no statistically significant difference between these rates(p>0,05). Similarly the Oxidized Thiol, Disulfide / TT and Disulfide / NT results of the NAC group were found to be lower than the İİR + İL and İİR + İL + NAC groups, but there was no statistically significant difference between these rates(p>0,05). CONCLUSION: It was found that the use of Iloprost and N-Acetylcysteine in the IIR injury model was not superior to the use of N-Acetylcysteine alone. We think that Thiol-Disulfide hemostasis parameters can be used as an indicator of antioxidant mechanism in IIR damage and our results will contribute to new studies