A comparison of ?serum creatinine? and ?serum neutrophil gelatinase-associated lipocalin? levels for the early diagnosis of ?acute kidney injury due to experimental rhabdomyolisis created in rats

INTRODUCTION and OBJECTIVES The duration of the diagnosis of acute kidney injury prolongs since the earliest increase in serum creatinine is within 24 hours and acute kidney injury does not exhibit without 50% loss of kidney function. Neutrophil gelatinase-associated lipocalin is a new biomarker for the early stage diagnosis of acute kidney injury. In our study, we aimed to investigate the value of "Serum neutrophil gelatinase-associated lipocalin" and "serum creatinine" in the early stage of diagnosis for acute kidney injury due to an experimental rhabdomyolysis induced by intramuscularly injection of glycerol.MATERIALS AND METHODS Tail veins of rats under ether anesthesia were cannulated with 24 gauge branule and baseline blood samples were collected. Rats were divided into two groups as study and control groups. 2 ml of 50% glycerol was injected via intramuscularly to rats in study group (n=7). No additional procedure was done for the control group (n=7). For neutrophil gelatinase-associated lipocalin and serum creatinine levels, blood samples were collected in the second and 24th hour from tail vein again from each group. Left unilateral nephrectomy was performed for pathologic evaluation. At the end of the study, rats were sacrificed under high dose ether anesthesia.RESULTS Acute kidney injury developed in all of study group while no acute kidney injury developed in the control group. In both of two groups, no increase was determined for the levels of neutrophil gelatinase-associated lipocalin in the second and 24th hour compared to the baseline levels.CONCLUSION In this experimental rat model, serum neutrophil gelatinase-associated lipocalin (NGAL) levels were found insignificant for the early diagnosis of acute renal injury induced by intramusculary injection of glycerol in rats.KEY WORDS Acute Kidney Injury, Neutrophil Gelatinase-Associated Lipocalin (NGAL), Emergency Medicine, Contrast Nephropathy, Rabdomyolozis, Crush Syndrome